Homelessness & Adverse Childhood Experiences: The Health and Behavioral Health Consequences of Childhood Trauma

This fact sheet was developed by the National Health Care for the Homeless Council and the National Network to End Family Homelessness, an initiative of The Bassuk Center on Homelessness and Vulnerable Children and Youth. The purpose is to ensure clinicians working with people experiencing homelessness understand the role of Adverse Childhood Experiences (ACEs) in health outcomes as well as the options for responding

Iron-Catalyzed Oxidative α-Amination of Ketones with Primary and Secondary Sulfonamides

We report the iron-catalyzed α-amination of ketones with sulfonamides. Using an oxidative coupling approach, ketones can be directly coupled with free sulfonamides, without the need for prefunctionalization of either substrate. Primary and secondary sulfonamides are both competent coupling partners, with yields from 55% to 88% for deoxybenzoin-derived substrates

In Situ Quantification of Surface Chemistry in Porous Collagen Biomaterials

Cells inside a 3D matrix (such as tissue extracellular matrix or biomaterials) sense their insoluble environment through specific binding interactions between their adhesion receptors and ligands present on the matrix surface. Despite the critical role of the insoluble matrix in cell regulation, there exist no widely-applicable methods for quantifying the chemical stimuli provided by a matrix to cells. Here, we describe a general-purpose technique for quantifying in situ the density of ligands for specific cell adhesion receptors of interest on the surface of a 3D matrix. This paper improves significantly the accuracy of the procedure introduced in a previous publication by detailed marker characterization, optimized staining, and improved data interpretation. The optimized methodology is utilized to quantify the ligands of integrins α[subscript 1]β[subscript 1], α[subscript 2]β[subscript 1] on two kinds of matched porous collagen scaffolds, which are shown to possess significantly different ligand density, and significantly different ability to induce peripheral nerve regeneration in vivo. Data support the hypothesis that cell adhesion regulates contractile cell phenotypes, recently shown to be inversely related to organ regeneration. The technique provides a standardized way to quantify the surface chemistry of 3D matrices, and a means for introducing matrix effects in quantitative biological models.National Institutes of Health (U.S.) (Grant RO1 NS051320)National Institutes of Health. National Institute for Biomedical Imaging and Bioengineering (Biomechanics Training Grant T32EB006348)National Science Foundation (U.S.). Graduate Research Fellowship Program (Grant DGE-1122374)Singapore-MIT Alliance for Research and Technology (SMART). BioSym IRGComputation and Systems Biology Programme of Singapore--Massachusetts Institute of Technology AllianceNational Institutes of Health (U.S.) (Grant 9P41EB015871-26A1

Relationship Talk With Partners and Friends During Emerging Adulthood: The Role of Relationship Satisfaction

Research indicates that discussing one’s romantic relationship with one’s partner benefits individual well-being and reduces uncertainty about the future of the relationship. Implications of relationship talk with friends remain less clear, though talking with friends may actually increase uncertainty about the relationship (e.g., by making one’s partner jealous of these friends), particularly for emerging adults. Relationship talk with friends may be especially likely to promote relational uncertainty for couples who are already unsatisfied in their relationships. In this study, we explored whether relationship talk with one’s partner and one’s friends would each be uniquely associated with depressive symptoms and uncertainty about the relationship, specifically in the form of perceived partner jealousy of one’s friends and whether these associations would be moderated by relationship satisfaction. Results from a series of path models using data from 202 romantically involved emerging adults in the United States revealed that associations between relationship talk and outcomes were indeed moderated by relationship satisfaction. For example, only in unsatisfied relationships was relationship talk with friends positively associated with a partner\u27s jealousy and negatively associated with depressive symptoms. This research expands our understanding of relationship talk by differentiating between talk with partners versus friends, while considering the contextual role of relationship satisfaction

Potential Biomarkers for Physical Exercise-Induced Brain Health

Physical exercise has long been recognized as an effective and economic strategy to promote brain health in humans. The cellular and structural changes in the brains of exercised animals, including enhancements of neurogenesis and synaptogenesis, dendritic remodeling, and synaptic plasticity, have been considered as the key biological alterations accounting for exercise-elicited benefits to brain health. However, what transduces body movements into the above-mentioned changes remains largely unknown. Emerging theories indicate that physical activity triggers the release of various factors into the circulation from skeletal muscle (neurotrophins, myokines, and cytokines) and/or adipose tissue (adipokines). In this chapter, we review several of these molecules that are potentially implicated in this process, including neurotrophic factors (BDNF, IGF-1, and VEGF), adipokines (adiponectin and irisin), and myokines/cytokines (IL-15). The relationship, either causal or concomitant, between levels of these molecules (particularly in the blood) and brain function after exercise may help to identify biomarkers that can serve as objective indicators to evaluate exercise therapy on diseased or ageing brain. In addition, unmasking biomarkers may be instrumental in elucidating the mechanisms mediating exercise-induced brain health, thereby contributing to novel drug discovery for treatments to maintain brain health

Trauma Masa Anak, Hubungan Romantis, dan Kepribadian Ambang

Orang dengan kepribadian ambang (KA) ditandai dengan impulsif, emosional tidak stabil, memiliki aktivitas seksual yang tidak aman, dan tindakan lain yang berisiko untuk melakukan bunuh diri. Meskipun beberapa perilaku tidak termasuk dalam kejahatan, tetapi jika tidak ada pencegahan, maka KA akan membahayakan dirinya dan lingkungannya. Penelitian ini bertujuan untuk mengetahui hubungan trauma masa kecil, hubungan romantis, dengan KA. Hipotesisnya adalah 1) ada hubungan antara trauma masa kanak-kanak dan KA; 2) ada hubungan antara hubungan romantis dan KA. Partsipan dalam penelitian ini 77 istri berusia 20-40 tahun. Data dikumpulkan dengan menggunakan tiga skala, yaitu Skala Kepribadian Ambang, Skala Trauma Masa Anak, dan Skala Hubungan Romantis. Analisis data menggunakan product moment. Hasil penelitian ini, 1) ada hubungan positif yang signifikan antara trauma masa kanak-kanak dan kepribadian ambang (r = 0.6, p <0,01); 2) ada hubungan negatif yang signifikan antara hubungan romantis dan kepribadian ambang (r = - 0.5 = p <0,01). Kata kunci: hubungan romantis; kepribadian ambang; trauma masa ana

Pre-treatment HIV-drug resistance associated with virologic outcome of first-line NNRTI-antiretroviral therapy: A cohort study in Kenya

Background: Pre-treatment HIV-drug-resistance (PDR) to WHO-recommended 1st-line non-nucleoside reverse transcriptase inhibitors (NNRTI)-based antiretroviral treatment (ART) is increasing in low-resource communities. We evaluated the risk of PDR on treatment failure if detected at single or multiple codons, at minority (2–9%) or higher (≥10%) frequencies during efavirenz- vs. nevirapine-ART. Methods: We conducted a pooled analysis across three cohorts of Kenyans initiating 1st-line NNRTI-ART between 2006 and 2014. Mutations K103N, Y181C, G190A, M184V and K65R were detected by an oligonucleotide ligation assay (OLA) and confirmed by Sanger and next-generation sequencing (NGS). PDR was defined as detection of any mutation by OLA when confirmed by NGS. Treatment failure, defined as plasma HIV RNA ≥400 copies/mL at month-12 of ART, was compared by PDR genotypes. Findings: PDR was detected in 59/1231 (4·8%) participants. Compared to wild-type genotypes, PDR in participants prescribed nevirapine-ART was associated with increased treatment failure [PDR 69·2% (27/39) vs. wild-type 10·4% (70/674); p = 0·0001], whether detected as minority [66·7% (4/6)] or higher [69·7% (23/33)] frequencies in an individual\u27s HIV quasispecies (p = 0·002 and p \u3c 0·0001, respectively), or mutations at single [50·0% (12/24)] or multiple [100·0% (15/15)] codons (p \u3c 0·0001). During efavirenz-ART, PDR was also associated with increased virologic failure [PDR 25·0% (5/20) vs. wild-type 5·0% (25/498); p = 0·005], but only if detected at multiple drug-resistant codons [50·0% (3/6); p = 0·003] or high frequencies PDR [33·3% (5/15); p = 0·001]. Interpretation: The risk that PDR confers for treatment failure varies by number of mutant codons and their frequency in the quasispecies, with a lower risk for efavirenz- compared to nevirapine-based regimens. PDR detection and management could extend the effective use of efavirenz-ART in low-resource settings. Funding: NIH, PEPFAR

Which objective sleep elements predict children’s perceptions of good sleep quality? A preliminary investigation based on polysomnography and actigraphy

Objectives: Objective sleep elements that underlie child ratings of sleep quality are largely unknown. Child-based sleep recommendations, therefore, typically focus on duration. An expert panel recently provided specific recommendations regarding objective sleep parameters that correspond with higher quality sleep, but child-based studies from which to draw conclusions were notably limited. The present study used actigraphy and polysomnography to explore sleep continuity and architectural variables that correspond with higher ratings of sleep quality in a sample of school-aged children.  Methods: Fifty-two healthy, prepubertal children (aged 7–11 years) completed one night of unattended ambulatory polysomnography at home with concurrent actigraphy and provided sleep quality ratings the following morning. Associations between sleep variables and subjective ratings were examined using polynomial regression models to examine potential linear and nonlinear relationships.  Results: In contrast to findings among adults, total sleep time, sleep onset latency, and sleep efficiency values were unrelated to child ratings of sleep quality. Wake after sleep onset (WASO) showed a curvilinear (reversed j-shaped) relationship such that perceptions of sleep quality were high when WASO values were less than approximately 30 minutes. For sleep architecture, N1% showed a significant quadratic association with sleep quality such that N1% between 2% and 6% corresponded with high sleep quality ratings.  Conclusions: Our findings support expert recommendations regarding WASO values that predict high quality sleep in children, but also await replication. There is need for additional research aimed at understanding objective sleep elements and other influences of children's perceptions of sleep quality using linear and nonlinear models